primary antibody psd93  (Developmental Studies Hybridoma Bank)

Journal: bioRxiv

Article Title: Persistent cortical excitatory neuron dysregulation in adult Chd8 haploinsufficient mice

doi: 10.1101/2025.06.04.657776

Figure Lengend Snippet: A) Barplot of DEG counts across bulk RNA-seq experiments (constitutive Chd8 +/- , Camk2a-CRE conditional Chd8 +/flox , and nuclear and synaptosomal preparations from the Chd8 +/- line. B) Chd8 is down-regulated in all but the nuclear preparation. C) LogFC concordance between constitutive and conditional datasets for constitutive model DEGs. D) LogFC concordance plots for nuclear and synaptosomal versus whole cortex for DEGs identified in nulear or synaptosomal fractions on left, reduced concordance between nuclear and synapsomal DEG logFC on right. E) Selected enriched GO terms for upregulated (left) and downregulated (right) genes across experiments. F) Downregulation of Dlg2 in synaptosomal RNA. GH) Western blot validating decreased synaptosomal PSD93 protein in Chd8 +/- cortex.

Article Snippet: Primary antibody PSD93 (1:50, DSHB, Clone N18/28, supernatant fraction) was diluted in 7.5 ml Intercept PBS blocking buffer with 0.1% Tween.

Techniques: RNA Sequencing, Western Blot

Journal: Antioxidants

Article Title: Hyperbaric Oxygen Improves Cognitive Impairment Induced by Hypoxia via Upregulating the Expression of Oleic Acid and MBOAT2 of Mice

doi: 10.3390/antiox13111320

Figure Lengend Snippet: ( A ) Experimental timeline describing the time of exposing to CON, Hypoxia or HBO in all three groups (n = 10). ( B ) The weight of mice (n = 10). ( C ) Y maze spontaneous alternation. ( D ) The total number of arm entries of the Y maze (n = 6). ( E ) The escape latency. ( F ) The swimming trajectories. ( G ) The number of crossings over the original platform location of CON, Hypoxia, and HBO mice during the test period. The percentages of swimming ( H ) distance and ( I ) time in goal area. ( J ) The speed of exploration of CON, Hypoxia, and HBO mice during the test period (n = 7). ( K ) Western blots of PSD93 protein and corresponding quantitative results in the prefrontal cortex (n = 3). ( L ) Representative images of Nissl body staining (blue spots, 200×) and corresponding quantitative results in the prefrontal cortex (n = 3). Scale bar, 50 μm. ( M ) Representative images of NeuN staining (vivid green dots, 200×) and corresponding quantitative results in the prefrontal cortex (n = 3). The nucleus was stained with DAPI (blue color). Scale bar, 50 μm. Data are presented as mean ± standard error of the mean (SEM). The behavioral changes over time during the training period were analyzed by repeated measure ANOVA. Significant level: ns: not statistically significant, * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: The protein was separated with SDS-PAGE gel and transferred to PVDF membranes, and incubated overnight at 4 °C with the primary antibodies against: Glutathione Peroxidase 4 (GPX4) (proteintech), Solute carrier family 7 member 11 (SLC7A11) (proteintech), Postsynaptic Density 93 (PSD93) (HUABIO, Woburn, MA, USA).

Techniques: Western Blot, Staining

Journal: Antioxidants

Article Title: Hyperbaric Oxygen Improves Cognitive Impairment Induced by Hypoxia via Upregulating the Expression of Oleic Acid and MBOAT2 of Mice

doi: 10.3390/antiox13111320

Figure Lengend Snippet: Effects of MBOAT2 and OA on FPT and cognitive markers. ( A ) Survival rate of SY5Y cells under different concentration of OA. ( B ) Effect of overexpression of MBOAT2 plasmid. ( C ) The heat map showed the expression of FPT indicators in each group. As it approached pink, the content was higher, while as it approached blue, the content was lower. Levels of ( D ) lipid peroxidation, ( E ) MDA, ( F ) GSH in SY5Y cell between different groups (n = 3). ( G ) Western blots of GPX4, PSD93 protein and corresponding quantitative results in cell (n = 3). Significant differences were tested by one-way ANOVA. Significant level: * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: The protein was separated with SDS-PAGE gel and transferred to PVDF membranes, and incubated overnight at 4 °C with the primary antibodies against: Glutathione Peroxidase 4 (GPX4) (proteintech), Solute carrier family 7 member 11 (SLC7A11) (proteintech), Postsynaptic Density 93 (PSD93) (HUABIO, Woburn, MA, USA).

Techniques: Concentration Assay, Over Expression, Plasmid Preparation, Expressing, Western Blot